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csytan | 6 years ago
> Virus stability in aerosols was determined as described previously at 65% relative humidity (RH) and 21-23°C. In short, aerosols (<5 µm) containing HCoV-19 (105.25 TCID50/mL) or SARS-CoV-1 (106.75-7 TCID50/mL) were generated using a 3-jet Collison nebulizer and fed into a Goldberg drum to create an aerosolized environment. Aerosols were maintained in the Goldberg drum and samples were collected at 0, 30, 60, 120 and 180 minutes post-aerosolization on a 47mm gelatin filter (Sartorius). Filters were dissolved in 10 mL of DMEM containing 10% FBS. Three replicate experiments were performed.
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