These vectors contain a number of indicator genes, such as antibiotic resistance, so you can use the antibiotic to kill the bacteria that didn't take up your vector.
The vectors have a prepared insertion site to take your gene, that's right in the middle of another gene that produces a colored product.
If the your resultant bacteria produces the colored product, you know you gene didn't make it into the insertion site or it would have broken that gene.
There's vast catalogues of this stuff.
Then when you want to do full genetic sequence to see where your gene has inserted, that's pretty much automated for you.
If your experience is anything like mine, once you start searching for this, google ads is going to lure you into the rabbit hole with offers to 'automate your agrigenomic high throughput whole sequence workflow' and 'rapid de novo genome assembly'
Some of the stuff google is trying to sell me, seems only slightly more non-fictional than 'mystery flesh pit national park', which is of course a searchable phrase.
monkeycantype|4 years ago
As I said i'm not an expert, just a first year student getting my mind blown.
But in my classes, we look at catalogs of genetic vectors:
here's a top google hit https://en.vectorbuilder.com/
These vectors contain a number of indicator genes, such as antibiotic resistance, so you can use the antibiotic to kill the bacteria that didn't take up your vector.
The vectors have a prepared insertion site to take your gene, that's right in the middle of another gene that produces a colored product.
If the your resultant bacteria produces the colored product, you know you gene didn't make it into the insertion site or it would have broken that gene.
There's vast catalogues of this stuff.
Then when you want to do full genetic sequence to see where your gene has inserted, that's pretty much automated for you.
https://www.thermofisher.com/au/en/home/life-science/sequenc...
You want to compare the genetic sequence to other organisms, there are online search engines for that:
https://blast.ncbi.nlm.nih.gov/Blast.cgi
If your experience is anything like mine, once you start searching for this, google ads is going to lure you into the rabbit hole with offers to 'automate your agrigenomic high throughput whole sequence workflow' and 'rapid de novo genome assembly'
Some of the stuff google is trying to sell me, seems only slightly more non-fictional than 'mystery flesh pit national park', which is of course a searchable phrase.