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carreau | 2 years ago

Even on classical 2D microscope the illumination can be non-uniform, and you might need to calibrate your image.

Source: PhD in biolab with microscopes, and napari dev.

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dekhn|2 years ago

OME-zarr doesn't really storage per-pixel illumination data. Instead, the illumination will typically be storaged as a per-2D-plane metadata.

Flat fields, dark fields, and light fields can all be stored but would be their own arrays (structure of arrays rather than array of structures).