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abcc8 | 1 year ago

When I did the same, also 15 years ago, the lab on campus would only Sanger sequence my insert - and for targets larger than ~600bp I also had to include internal sequencing primers (that had to be ordered separately, shipped, etc). Plasmidsaurus will return the entire plasmid sequence so you can verify it is intact, you don't have to provide any internal sequencing primers, simple repeats will be more accurately sequenced, and you can just pop some tubes in a padded mailer in the post at room temperature.

I'm no longer doing wet bench work but Plasmidsaurus seems like a service I'd happily use.

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