It is an expensive research project. Actually doing this starting from no experience or tools you're probably looking at 6 months - 1 year and 10k. A little support from trained biologists would help a lot though.
Using DIY tools and not being a trained biostatistician or whoever usually looks at these things you are very likely to face errors you don't know how to account for. I would guess the odds are high you'll encounter scary false positives for example.
It sounds really fun though, something I've always wanted to do with more time.
greazy|5 months ago
Even then, you'll need expertise in analysing the output reads. And EVEN then it's difficult to accurately assess variants.
leoh|5 months ago
a_tartaruga|5 months ago
Using DIY tools and not being a trained biostatistician or whoever usually looks at these things you are very likely to face errors you don't know how to account for. I would guess the odds are high you'll encounter scary false positives for example.
It sounds really fun though, something I've always wanted to do with more time.