[koeng]

Already answered there: I’m using bakers yeast, not lab yeast (store-bought S cerevisiae). It’s not haploid, often it’s tetraploid. HR doesn’t guarantee homozygous transformation.

Same answer for electroporation vs spheroplasty. I’ve found with wild yeasts or less tamed yeasts (pichia), sometimes just nuking the damn thing with kV will just work, whereas those chemical methods can be way more finicky. Time is money