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pcrh | 23 days ago

I briefly scanned the paper. The above summary is garbage.

For a biologist, a summary might be like this: pcr fragments are generated with short reverse complementary sequences added to the end of one fragment that match that at the begining of the next to-be-joined fragment.

These will anneal to create a cross-shaped DNA molecule. The short arms of the cross being the complementary sequences. Like so:

  ======∥=====

The short arms can then be processed-off to leave behind the now-longer fragment. The process can be repeated using different reverse complementary sequences between each fragment, the "page numbers" referred to.

discuss

order

oofbey|23 days ago

So do the complementary sequences naturally bind to their neighbors? So you just mix the “pages” in a soup for a while until they all find their friends. And then the custom enzyme (or what is it) just slices off the three way junctions?

Really clever.

pcrh|23 days ago

That's right.

It's one of those elegant solutions that just seem so obvious once they're presented. But this lot did it first.