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pcrh | 23 days ago
For a biologist, a summary might be like this: pcr fragments are generated with short reverse complementary sequences added to the end of one fragment that match that at the begining of the next to-be-joined fragment.
These will anneal to create a cross-shaped DNA molecule. The short arms of the cross being the complementary sequences. Like so:
======∥=====
The short arms can then be processed-off to leave behind the now-longer fragment. The process can be repeated using different reverse complementary sequences between each fragment, the "page numbers" referred to.
oofbey|23 days ago
Really clever.
pcrh|23 days ago
It's one of those elegant solutions that just seem so obvious once they're presented. But this lot did it first.